Table 2.
Interference Testing at High Concentration of PCDH-Based Pseudovirus
| Reagent manufacturer |
N gene |
ORF1ab gene |
E gene |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control |
2 mg/L DNA |
P value | Control |
2 mg/L DNA |
P value | Control |
2 mg/L DNA |
P value | |||||||
| Mean Ct | Positive rate | Mean Ct | Positive rate | Mean Ct | Positive rate | Mean Ct | Positive rate | Mean Ct | Positive rate | Mean Ct | Positive rate | ||||
| PerkinElmer | 32.07 | 20/20 | 31.79 | 20/20 | 0.26 | 31.62 | 20/20 | 31.70 | 20/20 | 0.39 | |||||
| Wantai | 33.33 | 20/20 | 33.16 | 20/20 | 0.21 | 33.46 | 20/20 | 33.13 | 20/20 | 0.06 | |||||
| KHB | 28.53 | 20/20 | 28.41 | 20/20 | 0.03 | 29.92 | 20/20 | 30.02 | 20/20 | 0.38 | 28.52 | 20/20 | 28.34 | 20/20 | 0.02 |
| Sansure | 36.85 | 20/20 | 36.65 | 20/20 | 0.44 | 37.45 | 20/20 | 37.87 | 20/20 | 0.14 | |||||
| Da An | 35.58 | 20/20 | 35.78 | 20/20 | 0.20 | 37.26 | 20/20 | 37.37 | 20/20 | 0.46 | |||||
| BioGerm | 34.56 | 20/20 | 34.46 | 20/20 | 0.70 | 34.89 | 20/20 | 34.32 | 20/20 | 0.11 | |||||
| ABT | 36.02 | 20/20 | 36.15 | 20/20 | 0.23 | 36.99 | 20/20 | 36.16 | 20/20 | 0.01 | 35.55 | 20/20 | 35.55 | 20/20 | 0.89 |
The concentration was 900 copies/mL of N gene, 760 copies/mL of ORF1ab gene, and 670 copies/mL of E gene.
ABT, Beijing Applied Biological Technologies Co, Ltd; BioGerm, Shanghai BioGerm Medical Biotechnology Co, Ltd; Da An, Da An Gene Co, Ltd; KHB, Shanghai Kehua Bio-Engineering Co, Ltd; Sansure, Sansure Biotech Inc.; Wantai, Beijing Wantai Biological Pharmacy Enterprise Co, Ltd.