Figure 1).

A: Viral injection into medial prefrontal cortex. B: human-alpha-synuclein (α-syn) transfection in medial prefrontal cortex (magenta). C: Representative dendrites from basilar layer II/III and Layer V neurons stained using Golgi kit. D-L: Co-staining for human α-syn (magenta, bottom row), phospho-S129 α-syn (cyan, middle row) and composite images (D, G, J). G-I: At the injection site, both total α-syn (I) and phospho-α-syn (H) can be found in the nucleus, soma, dendrites, dendritic spines and axons, although phosphor-α-syn is highest in nucleus (H). J-L: One millimeter away from injection site, only axons are stained with human α-syn (L), and phospho-α-syn is absent (K). L-Q: Phospho-α-syn (cyan) and mCherry (red), from an animal co-injected with both control and α-syn virus (P-R) vs. control virus alone (S-U). PrL (prelimbic), IL (infralimbic) and Cg1 (cingulate).