Table 1.
Summary of antibodies and metal tags used for mass cytometry.
| Summary of monoclonal antibodies and metal tags used for mass cytometry | ||||
|---|---|---|---|---|
| TARGET | SPECIES | CLONE | ISOTYPE | MANUFACTURER |
| CD45 | Human | HI30 | 89Y | Fluidigm |
| CD3 | Human | UCHT1 | 154Sm | Fluidigm |
| CD4 | Human | SK3 | 174Yb | Fluidigm |
| CD8 | Human | SK1 | 168Er | Fluidigm |
| CD25 | Human | 2A3 | 149Sm | Fluidigm |
| CD45RA | Human | Hl100 | 169Tm | Fluidigm |
| CCR7 | Human | G043H7 | 159Tb | Fluidigm |
| CD95 | Human | DX2 | 164Dy | Fluidigm |
| NKG2D | Human | 1D11 | 166Er | BD Biosciences |
| ICOS | Human | C398.4A | 148Nd | Fluidigm |
| CXCR3 | Human | G025H7 | 163Dy | Fluidigm |
| 4-1BB | Human | 4B4-1 | 173Yb | Fluidigm |
| OX40 | Human | ACT35 | 150Nd | Fluidigm |
| LAG-3 | Human | 11C3C65 | 165Ho | Biolegend |
| Ki67 | Cross | B56 | 161DY | Fluidigm |
Preconjugated antibodies were purchased from Fluidigm. All other antibodies were purchased in carrier-protein-free PBS and conjugated with the respective metal isotype using the MaxPAR antibody conjugation kit (Fluidigm) according to manufacturer’s instructions. Metal-labeled antibodies were diluted to 0.5 mg/ml in Candor PBS Antibody Stabilization solution (Candor Bioscience GmbH) for long-term storage at 4°C.