FIGURE 11.

Inhibition of LINC01559 suppressed PC cell autophagy and promoted apoptosis. (A) Protein expression levels of p62 and LC3B after various treatments measured by Western blot analysis. (B) Representative confocal images of autophagosome (yellow puncta) and autolysosome (red puncta) formation are presented in the left panel. Scale bar: 20μm. The numbers of RFP⁺GFP⁺ LC3 puncta and RFP⁺GFP^– LC3 puncta are shown in the right panel. (C) The cell inhibition was calculated by the CCK-8 assay in PC cells treated with different concentrations of gemcitabine at 48 h. (D) Representative images from the clonogenic assays of PANC-1 and SW1990 cells with or without gemcitabine (10 μM) treatments and cultured for 14 days. (E) Apoptosis rate after various treatments was detected by flow cytometry. (F) Protein expression levels of cleaved PARP and cleaved caspase-3 after various treatments measured by Western blot analysis (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant).