Figure 4.
Communication deficits in Flr mice. A, Representative spectrograms of a P8 pup during maternal separation. B, Maternal isolation induced USV on P8 and P10 (n = 10 for both WTFLR and Flr). Flr showed statistically significant increase in total duration of calls. C, Representative spectrogram of P8 pup USV during cold-temperature-induced vocalization. D, Cold temperature-induced USV (n = 10 for both WTFLR and Flr). Flr showed statistically significant increase in total duration of calls on P8. Unpaired non-parametric t test was used for statistical analysis. Error bars represent ±SEM; *p ≤ 0.05, **p ≤ 0.001.