Figure 2. RF ON and OFF subfields measured by flashed spots are spatially offset.
a, Average responses of a F-mini-ON RGC to 30 μm spots of positive (orange) or negative (black) contrast at the indicated positions. b,c, Interpolated ON and OFF spatial RFs from the data in a. White and black crosses mark the ON and OFF centers-of-mass (COM), respectively. d, Population data showing the fractional overlap of the OFF RF relative to the ON RF for F-mini-ON RGCs (magenta) and other ON-OFF RGCs (grey) (n = 9, 14 cells, see Methods). e, Polar histograms showing the offset angle between the ON RF COM and the OFF RF COM for F-mini-ON RGCs (magenta) and other ON-OFF RGCs (grey). OFF ventral of ON is shown as a downward (ventral) angle. f, Image of the cell from a-c overlaid with its ON (orange) and OFF (black) RF contours. g, Average OFF RF for F-mini-ON RGCs aligned to the center-of-mass of each ON RF at the origin. Points are the center-of-mass for individual cells (n = 9). Scale bar is the same in a-c,f,g. Box plots in d show maximum, 75th percentile, median, 25th percentile, and minimum.
