Table 1.
Number of mosquito samples processed for midgut bacteria.
| Site | Mosquito species | Number of midgut samples | |
|---|---|---|---|
| Initial | Final | ||
| Lingshui (LS) | Aedes galloisi (AGA) | 34 | 30 |
| Aedes albopictus (AAL) | 39 | 30 | |
| Tunchang (TC) | Culex pallidothorax (CPA) | 25 | 18 |
| Aedes albopictus (AAL) | 30 | 27 | |
| Haikou (HK) | Culex pipiens (CPI) | 42 | 30 |
| Culex gelidus (CGE) | 13 | 11 | |
| Aedes albopictus (AAL) | 35 | 30 | |
| Armigeres subalbatus (ASU) | 32 | 30 | |
| Dingan (DA) | Aedes albopictus (AAL) | 31 | 30 |
| Culex pallidothorax (CPA) | 16 | 15 | |
| Wenchang (WC) | Armigeres subalbatus (ASU) | 32 | 30 |
| Aedes albopictus (AAL) | 33 | 30 | |
| Culex pallidothorax (CPA) | 19 | 14 | |
| Sanya (SY) | Aedes albopictus (AAL) | 34 | 30 |
| Armigeres subalbatus (ASU) | 30 | 29 | |
| Wuzhishan (WZS) | Culex pipiens (CPI) | 29 | 27 |
| Armigeres subalbatus (ASU) | 31 | 30 | |
| Aedes albopictus (AAL) | 32 | 30 | |
| Ledong (LD) | Aedes albopictus (AAL) | 32 | 30 |
| 569 | 501 | ||
Each female mosquito was surface sterilized in 75% ethanol for 10 min, then rinsed three times in sterile PBS solution. A total of 11–30 midguts were dissected for each mosquito species, and the samples were evenly distributed, and three groups were repeated.