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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Bone. 2020 Nov 4;143:115733. doi: 10.1016/j.bone.2020.115733

Figure 6. Jab1 deficiency inhibited osteoblast differentiation and mineralization ex vivo.

Figure 6.

(A) Left and middle panels: Jab1 RNA and protein expression levels in control and Jab1-knockdown mouse primary calvarial osteoblasts. Right panel: von Kossa staining in control and Jab1-knockdown mouse primary calvarial osteoblasts after 21 days of culture in osteoblast differentiation media. (B) Left and middle panels: Jab1 RNA and protein expression levels in control and Jab1-knockdown MC3T3-E1 cells. Right panel: von Kossa staining in Jab1-knockdown MC3T3-E1 cells after 21 days of culture in osteoblast differentiation media. (C) Left and middle panel: Jab1 RNA and protein expression levels in Jab1-knockdown mBMSCs. Right panel: oil red O staining of mBMSCs cultures.