Table 4.

The signal peptide results predicted by Signal-BLAST.

	Protein name	Sensitivity	Specificity	Balanced prediction	Cleavage site
Structural proteins	S protein	Yes	Yes	Yes	Yes
	Variant D614G	Yes	Yes	Yes	Yes
	E protein	No	No	No	Yes
	Variant L37H	No	No	No	No
	M protein	No	No	No	No
	Variant M T175M	No	No	No	No
	N protein	No	No	No	Yes
	Variant N P13L	No	No	No	Yes
	Variant N S194L	No	No	No	Yes
	Variant NS197L	No	No	No	Yes
	Variant N R203K/G204R	No	No	No	Yes
Non-structural proteins	orf3a	No	No	No	No
	orf6	No	No	No	Yes
	orf7a	Yes	Yes	Yes	Yes
	ORF7b	No	No	No	Yes
	orf8	Yes	Yes	Yes	Yes
	Variant orf8 S24L	Yes	Yes	Yes	Yes
	Variant orf8 V62L	Yes	Yes	Yes	Yes
	Variant orf8 L84S	Yes	Yes	Yes	Yes
	orf10	No	No	No	Yes
Pp1ab	nsp-1	No	No	No	Yes
	nsp-2	No	No	No	Yes
	nsp-3	No	No	No	No
	nsp-4	No	No	No	Yes
	nsp-5	Yes	No	Yes	Yes
	nsp-6	Yes	No	Yes	Yes
	nsp-7	No	No	No	Yes
	nsp-8	No	No	No	No
	nsp-9	Yes	No	Yes	Yes
	nsp-10	Yes	Yes	Yes	Yes
	nsp-12	No	No	No	Yes
	nsp-13	No	No	No	No
	nsp-14	No	No	No	No
	nsp-15	No	No	No	No
	nsp-16	No	No	No	No

Bold indicates proteins that have a signal peptide.