Fig1. depletion attenuates tumor growth in syngeneic mice.
About 1 × 105 vector control and PCSK9 knockout murine tumor cells were inoculated subcutaneously into syngeneic mice and observed for tumor formation. Both tumor size and overall survival were monitored. a-b. 4T1 breast cancer line grown in Balb/c mice. n=9 and 20 mice for control and PCSK9KO tumor cells, respectively. c-d. B16F10 melanoma line grown in C57BL/6 mice. n=12 mice for both groups. e-f. CT26 colon cancer line grown in Balb/c mice. n=5 mice for both groups. g-h. MC38 colon cancer line grown in C57BL/6 mice. n=5 mice for both groups. Error bars: mean ± S.E.M. P values were calculated by two-way ANOVA in a, c, e, g and log-rank test in b, d, f, h, respectively.