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. Author manuscript; available in PMC: 2021 Dec 23.
Published in final edited form as: Cell. 2020 Dec 2;183(7):1757–1771.e18. doi: 10.1016/j.cell.2020.11.005

Figure 6 – Xre proteins regulate components of RNA-guided transposition.

Figure 6 –

(a-b) Consensus sequence for putative Xre binding motifs in I-F3a and I-Fb elements.

(c-d) Xre-binding resolved by EMSA. DNA fragments with the transcription control regions were incubated with increasing amounts of Xre protein from the respective element before electrophoresis (100 nM DNA; protein:DNA ratios = 0,2,5,10,20:1). (e-f) Promoter function resolved by LacZ expression monitored by Miller units at various arabinose controlled Xre expression levels. Transcription control regions are indicated from V. cholerae HE-45 Tn6677 (Vc), V. parahaemolyticus RIMD221063 (Vp). A. salmonicida S44 Tn6900 (As), and Vibrio sp. 10N.286.45.B6 (VB6).