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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2020 Nov 20;130:105885. doi: 10.1016/j.biocel.2020.105885

Fig. 3.

Fig. 3.

TNFAIP8 activates the PI3K-AKT pathway and induces cell proliferation. (A) Stable expression of TNFAIP8-Myc tagged protein in PC3 and LNCaP cells were analyzed by western blotting. (B) The effects of TNFAIP8 stable expression on AKT-mTOR pathway proteins were analyzed by western blotting. (C) ActivSignal IPAD assay. The impact of stable EV or TNFAIP8 expression on the regulation of PI3K-AKT pathway proteins and phosphorylation of key PI3K-AKT pathway proteins were analyzed from PC3 and LNCaP cells. The signal was normalized to the expression of housekeeping gene β-actin and plotted. *P<0.05, **P<0.01, ***P<0.001 compared to EV stable transfected cells. (D) RWPE1, PC3, LNCaP cells were transfected with EV or TNFAIP8-Myc tagged plasmid (2 μg) for 30h, and expression of TNFAIP8-Myc tagged protein and pS2448-mTOR, mTOR, pS473-AKT and AKT were analyzed by western blotting.