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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2020 Nov 20;130:105885. doi: 10.1016/j.biocel.2020.105885

Fig. 4.

Fig. 4.

TNFAIP8 regulates glucose metabolizing enzyme expression and glucose consumption in PCa cells. (A) RWPE1 and PCa cells (1×105) were grown in six-well plates 24h and transfected with EV or TNFAIP8 plasmid for 30h. The expression of TNFAIP8 mRNA levels was analyzed by RT/qPCR. (B) Similarly, the effect of TNFAIP8 expression on the regulation of glucose metabolizing enzymes (HK1, HK2, GLUT1, G6PD, AMPK, LDHK, PEPCK, and IL-6) mRNA expression was analyzed by RT/qPCR as described in the materials and methods section. (C) RWPE1, PC3, and LNCaP (1×105) cells were grown in six-well plates 24h and transfected with EV or TNFAIP8-myc plasmid for 30h. The cell lysates were immunoblotted with Hexokinase 1 (HK1), Glut1, G6P, AMPKα, and GAPDH antibodies. (D) RWPE1, PC3, and LNCaP (1×105) cells were grown in 6-well plates for 24h and transfected with EV or TNFAIP8-myc plasmid for 40h and the effect of TNFAIP8 expression on glucose consumption and endogenous ATP production was determined (upper and lower panels). Data represent mean ± SEM from three independent experiments. *P<0.05, **P<0.01,***P<0.001 compared to EV transfected or control cells.