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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2020 Nov 20;130:105885. doi: 10.1016/j.biocel.2020.105885

Fig. 5.

Fig. 5.

TNFAIP8 knockdown reduced glucose consumption and ATP production in PCa cells. (A) RWPE1, PC3, and LNCaP (1×105) cells were grown in six-well plates 24h and transfected with control siRNA or TNFAIP8 siRNA. Cell lysates from control siRNA or TNFAIP8 siRNA transfected cells were immunoblotted with TNFAIP8, HK1, AMPKα, and β-actin antibodies. (B) RWPE1, PC3, and LNCaP (1×105) cells were grown in six-well plates 24h and transfected with control siRNA or TNFAIP8 siRNA for 40h. The effect of TNFAIP8 knockdown on relative glucose consumption (upper panel) and endogenous ATP production (lower panel) was measured as described in the materials and methods sections. Data represent mean ± SEM from two independent experiments in triplicates. *P<0.05, **P<0.01, ***P<0.01 compared to control siRNA transfected or control cells. ns- not significant. (C) RWPE1, PC3, and LNCaP (1×105) cells were grown in six-well plates 24h and pre-treated with 2-DG (5mM) for 8h, and then cells were transfected with EV or TNFAIP8 plasmid for 40h and relative cellular glucose consumption was analyzed as described in material and methods section. Data represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 compared to EV or TNFAIP8 transfected cells.