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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Stroke. 2020 Nov 9;52(1):274–283. doi: 10.1161/STROKEAHA.120.032766

Figure 1. Waves of calcium activity in the ischemic brain.

Figure 1.

A, Low magnification recording (0.2 Hz) acquired in the cortical parenchyma. Left panels show cells labeled with tdTomato in the Iba1-Cre; PC∷G5-tdT mouse. The panels on the right show the progression of calcium waves triggered by ischemic injury. The wavefront is contoured with a dotted white line. B, Higher power recording in the meningeal plane, consisting of meningeal macrophages. Macrophages did not display marked calcium activity in response to the underlying mild calcium wave in the cortex. C, In contrast, microglia in the cortical plate showed strong calcium transients during the wave progression, especially in the later stages of ischemic injury. D, Diagram showing the distribution of calcium waves detected with two photon imaging in 3 stroke mice (MCAo 1-3) and 3 sham controls. The occurrence of waves is indicated with vertical ticks, the duration of imaging sessions is denoted with open boxes. A representative section of an infarcted brain and a control brain stained with TTC at the end of the imaging session are to the right of each group.