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. Author manuscript; available in PMC: 2022 Jan 8.
Published in final edited form as: J Mol Biol. 2020 Jun 29;433(1):166584. doi: 10.1016/j.jmb.2020.06.023

Figure 3. Representative B cell gating strategy.

Figure 3.

Splenic B220+ B cells (A) are differentially gated on IgM and CD21 to separate splenic B cells subsets into FO + T3, MZ + T2-MZP and T1 + T2 (B). C) T1 and T2 subsets are CD93+ and are separated by CD23 expression. D) MZ and T2-MZP subsets are separated by CD23 expression. E) FO B cells are separated from T3 by lack of CD93 expression. F) FO B cells are separated into IgDlow/− BDL and IgDhi FO B cells. The number on the contour plots indicates the percentage of cells in each gate.