Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 15;14:605312. doi: 10.3389/fncel.2020.605312

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Joksimovic, Evans, McIntire, Orestes, Barrett, Jevtovic-Todorovic and Todorovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

De-glycosylation with PNGase-F (PNG) causes depolarizing shift in voltage-dependent inactivation of Ca_V3.2 currents similar to NEU, but neither PNG nor NEU influence time course of recovery from inactivation. (A) When compared to untreated control cells (open symbols) treatment of HEK293 cells with PNG (gray symbols) induced significant shift of V₅₀ in steady-state inactivation curves from −71.6 ± 1.2 mV (n = 15) to −66.0 ± 2.2 mV (n = 7, p < 0.05). (B) Average graph from the experiments in 13 untreated (control) cells (open symbols), 4 cells treated with NEU (black symbols) and 7 cells treated with PNG (gray symbols) is shown on this panel. When compared with control conditions treatment with NEU or PNG minimally affected time course of recovery from inactivation of recombinant Ca_V3.2 currents. We used 10 mM Ba²⁺ as a charge carrier in these experiments.