Figure 2.

The development of alcohol tolerance in Drosophila. (A) Schematic of alcohol vapor exposure assay. Blue-dyed alcohol is applied to the vial plug, and following the first exposure, flies recover for 4 hours in fresh air. In the second exposure, at the same time point, fewer flies are sedated that in the first exposure, indicating that tolerance has developed. (B) Sample data shows ST50 (time it takes for 50% of the flies to become sedated) for a first and second alcohol vapor exposure for wildtype flies and flies with manipulations of different neurotransmitters. These manipulations are either genetic (“Reduced OA and TA” and “Reduced OA, Elevated TA”) or pharmacological via drug feeding (GABA agonist).