Skip to main content
. Author manuscript; available in PMC: 2021 Jun 9.
Published in final edited form as: Nature. 2020 Dec 9;588(7839):705–711. doi: 10.1038/s41586-020-2998-x

Extended Data Figure 11. Reelin improves cardio-protection in neonates and adult mice after MI.

Extended Data Figure 11.

a-d, Co-immunostaining using cell proliferation markers (EdU, Ki67, pH3 and AuroraB) together with the CM markers Prox1, αActinin or Mef2c shows decreased CM proliferation in the border of the infarcted area of Reln−/− hearts at P7. Arrows indicate proliferating CMs. N= 4 mice/group. e, Immunostaining using active Caspase-3 shows increased CM apoptosis in the infarcted area of Reln−/− hearts at P7. Arrows indicate apoptotic CMs in the section. N=4 mice/group. f, Immunostaining against the cell proliferation markers EdU, Ki67 and pH3 together with the CM markers Mef2c or cTnC shows no differences in CM proliferation in the infarcted areas between control patch or REELIN patch treated hearts 7 days after MI. Arrows indicate proliferating CMs. N = 4 hearts/group. g, Immunostaining using active Caspase-3 shows reduced CM apoptosis in the infarcted area of REELIN patch treated hearts. Arrows indicate apoptotic CMs. N=4 mice/group. Arrows indicate apoptotic CMs. Scale bars, 25 μm. Lower magnification for panels a-c, e and g are included in Supplementary Fig 3.