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. 2020 Dec 15;11:574229. doi: 10.3389/fphar.2020.574229

FIGURE 2.

FIGURE 2

SalB suppressed HFD-induced apoptosis of renal tubular cells in mice (A,B) Apoptosis (arrowheads) induced by HFD in kidney cells was detected by TUNEL assays (green) (magnification ×200). The corresponding quantitative analysis of TUNEL assays was shown as well. Nuclei were counterstained with Hoechst 33258 (blue) (C,D) Western blotting and the corresponding quantitative analysis showed HFD-induced decrease in Bcl-2 and increase in BAX and cleaved caspase-3 protein levels in the kidney cortex, which were ameliorated with SalB treatment. Nor, mice fed with normal diet; HFD, mice fed with high-fat diet; SalBL, SalBM, or SalBH, mice fed with high-fat diet and treated with low dosage of SalB (3 mg/kg), medium dosage of SalB (6.25 mg/kg), or high dosage of SalB (12.5 mg/kg), respectively. Data are represented as means ± SEM (n = 6). *p < 0.05 and **p < 0.01, compared with Nor; # p < 0.05 and ## p < 0.01, compared with HFD.