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. 2020 Dec 15;14:586107. doi: 10.3389/fnins.2020.586107

TABLE 1.

Summary of anti-SORT1 mAb characteristics.

Human U251 cell
 Mouse primary neuron
Clone Epitope bina hSORT1-His ELISAb (OD @450 nm) hPGRN-hSORT1 bindingc (% inhibition) SORT1 down-regulationd (% control) PGRN up-regulatione (fold-change) mSORT1-His ELISAf (OD @450 nm) SORT1 down-regulationg (% control) PGRN up-regulationh (fold-change) Functioni (PGRN competition or SORT1 down-regulation)
K1-19 I 1.44 13.2 95.4*** 2.40*** 0.78 4.2 1.13* SORT1 down-regulation
K1-32 I 1.48 22.7 95.7*** 2.20*** 0.83 15.7 1.14* SORT1 down-regulation
K1-52 I 0.19 80.5 44.1*** 1.10* 0.94 17.3 1.17*
K1-65 I 0.95 14.2 85.9*** 1.70** 0.53 19.6 1.19
K1-70 I 0.31 17.7 4.3 1.00 0.46 11.7 1.09*
K1-12 II 0.78 58.1 87.1*** 1.80*** 0.78 53.5** 2.32***
K1-66 II 0.14 34.6 31.9*** 1.00 0.19 16.6 1.40**
K1-15 III 0.70 90.7 71.1*** 2.00** 1.36 17.0* 2.49*** PGRN competition
K1-27 III 0.49 76.9 76.2*** 1.90* 1.43 28.8* 1.47**
K1-44 III 0.41 89.8 75.7*** 1.96*** 1.04 36.4** 1.83***
K1-54 III 0.54 99.5 75.1*** 1.90** 1.33 33.6* 1.74** PGRN competition
K1-62 III 0.22 96.3 13.7 1.00 0.15 5.4 1.14* PGRN competition
K1-68 III 0.38 86.1 80.1*** 2.00** 1.23 40.5* 1.65**
K1-11 IV 0.25 99.2 62.6*** 1.90** 1.17 35.7* 1.83* PGRN competition
K1-24 IV 0.16 27.1 63.8** 1.40** 0.23 46.7** 1.39**
K1-08 V 1.83 41.6 88.2*** 2.00*** 1.57 66.4** 1.80***
K1-09 V 1.97 47.2 84.7*** 1.60** 1.76 67.2** 1.78**
K1-13 V 1.91 31.9 95.0*** 1.90** 1.86 53.1** 1.55** SORT1 down-regulation
K1-40 V 2.01 14.5 94.1*** 2.30*** 1.90 65.5** 2.11*** SORT1 down-regulation
K1-47 V 0.32 22.1 7.4 0.90 0.43 9.8 1.06
K1-48 V 1.62 -3.5 95.4*** 1.80** 1.83 62.2** 2.29** SORT1 down-regulation
K1-61 V 1.42 33.6 85.1*** 1.60*** 1.17 53.9** 1.70**
K1-67 VI 1.00 63.0 95.2*** 1.90** 0.28 67.7*** 2.07*** SORT1 down-regulation
K1-71 VI 0.43 46.8 88.1*** 1.90** 0.14 37.9*** 1.28*
K1-02 VII 0.07 86.6 31.7** 1.10 1.37 43.1** 2.01**
K1-04 VII 0.37 95.0 58.9*** 1.60** 1.57 31.4* 1.78** PGRN competition
K1-05 VII 0.13 102.0 16.2** 1.00 1.37 36.4** 1.83*** PGRN competition
K1-06 VII 0.13 101.4 31.1** 1.10 1.62 40.2* 1.89** PGRN competition
K1-07 VII 0.17 100.0 12.1** 1.10 1.44 42.4** 1.70** PGRN competition

aEpitope bin was determined by competitive binding profiles of anti-SORT1 mAbs in sandwich ELISA. b,fBinding activity of anti-SORT1 mAb is shown as absorbance at 450 nm determined using ELISA. cPGRN-SORT1 interaction block by anti-SORT1 mAb was calculated as a % inhibition at 15 μg/mL (vs. PBS-treated control group). e,hPGRN up-regulation by anti-SORT1 mAb was calculated as a fold-change (vs. PBS-treated control). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. PBS-treated group by Student’s t-test.d,gSORT1 down-regulation is represented as SORT1 levels down-regulated from cell surface in response to anti-SORT1 mAb. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. PBS-treated group by Student’s t-test.iThe inhibition of the interaction between PGRN and SORT1 in more than 90% was classified as PGRN competition. The down-regulation of SORT1 in more than 90% was classified as SORT1 down-regulation.