Figure 1.

Comparing the detection of circulating CD19⁺ B-cells with LSFC versus HSFC (A). Absolute numbers of circulating CD19⁺ B-cells were analyzed with HSFC (x-axis) and correlated to LSFC (y-axis) with, respectively, a detection limit of 1*10³ B-cells/L and 1*10⁶ B-cells/L. Each dot represents one timepoint for one patient before and 2, 4, 12, 24, or 48 weeks after RTX treatment (B). Each line represents the absolute CD19⁺ B-cell count for an individual patient during RTX treatment measured with LSFC (C). Each line represents the absolute CD19⁺ B-cell count for an individual patient during RTX treatment measured with HSFC. In (B, C), the dotted line indicated the detection limit for LSFC (10⁶ cells/L). Gray area indicates 1–20 analyzed events. Red dotted line indicated the detection limit for HSFC (10³ cells/L).