Figure 5.

Antibodies mediating B. pertussis aggregation reduced A549 pro-inflammatory signaling. (A) Anti-FHA and anti-FIM polyclonal sera reduced NF-κB activation and A549 cell inflammatory response. Cytoplasmic NF-κB levels remains higher in presence of anti-FIM and anti-FHA sera compared to control sera. (B) Complementary analysis of pro-inflammatory cytokines and chemokines released in the culture supernatant in response to B. pertussis infection were significantly reduced by anti-FIM and anti-FHA compared to naive sera. Unpaired Student’s t-test. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 n ≥ 3 experiments.