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. 2020 Dec 15;11:592436. doi: 10.3389/fgene.2020.592436

Figure 1.

Figure 1

HOXA5 expression in breast cancer cells and tissues. (A) Comparison of HOXA5 expression in different cell lines. The total RNA was isolated from CCL228 (colon cancer), H358 (lung cancer) HeLa (cervical cancer), JAR (placental cancer), MDAMB231 (ER-negative breast cancer), T47D (ER-positive breast cancer), MCF7 (ER-positive breast cancer) cell lines and analyzed by RT-qPCR using primers specific to HOXA5 and GAPDH (loading control). HOXA5 expression relative to GAPDH is plotted. Bars indicate averages ± standard errors (n = 3). **denotes p < 0.0001 compared to HEK293F control cells (B–D) HOXA5 expression in breast cancer tissue. Tissue microarray slide (containing six cases of human breast cancer cases and respective adjacent normal breast tissue) was subjected to DAB staining with HOXA5 antibody. The HOXA5 expression level was quantified (C). A magnified view of case 3 is in (D).