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. 2020 Dec 15;11:592436. doi: 10.3389/fgene.2020.592436

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Copyright © 2020 Hussain, Deb, Chini, Obaid, Bhan, Ansari, Mishra, Bobzean, Udden, Alluri, Das, Brothers, Perrotti and Mandal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERα and ERβ enrichment on the HOXA5 promoter ERE in the presence of E2. The HOXA5 promoter contains two putative ERE regions (ERE1 and ERE2) near the transcription start site (A). E2-treated (nM E2 for 6 h) and control (untreated) MCF7 cells were analyzed by ChIP assay using antibodies against ERα, ERβ, and β-actin (control). The ChIP DNA was analyzed by regular PCR (agarose gel analysis, B) and qPCR (C) using HOXA5 promoter primers (ERE1 and ER2 regions). Bars indicate averages ± standard errors (n = 3). *denotes p < 0.0001 compared to ERα ChIP control; ^#denotes p < 0.0001 compared to ERβ ChIP control for both the EREs.