Table 1.
Primers used for cloning, RT-PCR, ChIP, and antisense experiments.
| Primers | Forward primers (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|
| PCR PRIMERS | ||
| GAPDH | CAATGACCCCTTCATTGACC | GACAAGCTTCCCGTTCTCAG |
| HOXA5 | ACTCATTTTGCGGTCGCTAT | TTGTAGCCGTAGCCGTACCT |
| HOXA5-ERE1 | CGAGTCCGGCTGAACGGCGG | TAGGCACCCAAATATGGGGTA |
| HOXA5-ERE2 | TTATTTCTCCAATTGGCTAAA | CCGGCGAGGATGCAGAGGAT |
| ERα | AGCACCCTGAAGTCTCTGGA | GATGTGGGAGAGGATGAGGA |
| ERβ | AAGAAGATTCCCGGCTTTGT | TCTACGCATTTCCCCTCATC |
| ANTISENSES | ||
| HOXA5 | GTCCCTGAATTGCTCGCTCA** | |
| ERα antisense | TCCCACCTTTCATCATTCCC** | |
| ERβ antisense | GCCACACTTCACCATTCCCA** | |
| Scramble | CGTTTGTCCCTCCAGCATCT** | |
| antisense | ||
**
Phosphorothioate antisense oligonucleotides.