Table 3.

Recent reports about fabrication strategies of nanoliposomes applied to substances of interest in drug delivery.

Synthesis technique	Encapsulated agent	Objective	Results	References
Thin-film hydration—sonication	calothrixin B	To test anticancer activity against lung and breast cell lines A549 and MCF-7	High entrapment efficiency, Control size distribution, increased stability	Yingyuad et al., 2018
Ethanol injection	Black seed oil (Nigella sativa)	To enhance oral bioavailability and improve therapeutic activity in small animal studies of analgesia	Improvement of analgesic activity and oral bioavailability. Sucrose and cholesterol exhibited to improve the encapsulation efficiency of black seed oil.	Rushmi et al., 2017
Reverse phase evaporation	Pomegranate extract	To carry an efficient amount of pomegranate extract to sperm via lecithin nanoliposome to protect sperm against lipid peroxidation	Protection of ram sperm during cryopreservation without adverse effects. Pomegranate formulation improved the quality of ram semen after thawing	Mehdipour et al., 2017
Supercritical fluid technology	Melatonin	To load melatonin in nanoliposomes as a delivery system in order to increase its oral bioavailability	Uniform size distribution. Slow release feature in early digestive stages and more thorough characteristics in later stages of simulated digestion	Zhang et al., 2017
Supercritical assisted Liposome formation (SuperLip)	Amoxicillin	Encapsulation of an antimicrobial agent for intravenous application	Inhibition growth of E. coli bacteria Encapsulation efficiency up to 84%	Trucillo et al., 2020
Depressurization of an Expanded Liquid Organic Solution (DELOS)	α-Galactosidase-A	To produce protein-nanoliposome for the treatment of Lysosomal storage disorders (LSD)	Enhanced enzymatic activity and intracellular penetration. Entrapment efficiency of 40%	Cabrera et al., 2016
Particles from gas saturated solution PGSS	–	To investigate the effect of pressure, depressurization rate and temperature on the characteristics of the final formulation	Quality of the vesicles depends on the dispersion of the phospholipid molecules prior to their reorganization during the processing	Zhao and Temelli, 2015
Depressurization of an Expanded Solution into Aqueous Media (DESAM)	–	To design and validate a new process for bulk liposome formation	Range size from 50 to 200 nm Polydispersity index below 0.29	Meure et al., 2009
Heating method	Plasmid DNA	To prepare anionic nanoliposomes without using any volatile organic solvent or detergent in order to test their morphology, stability and DNA incorporation efficiency	Good reproducibility, long-term stability and potential nano liposome production in large quantities.	Mozafari et al., 2002
Mozafari method	Polyunsaturated fatty acids (PUFAs): docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	To investigate the oxidation of bulk DHA and EPA incorporated into liposomes during cold (4 °C) storage	Enhancement of the oxidative stability of DHA and EPA in aqueous media when compared with bulk systems	Rasti et al., 2012