FIGURE 1.

Procedure to build the device to visualize beads in confocal microscope. A, Cut the tip at 5 to 6 mm from the top, B, C, using another tip, apply a small amount of superglue for glass into the cut section of the tip (D) place the cut tip at the center of a coverslip, apply some pressure with the back of the forceps for a few seconds, E, wait for the superglue to cure, small bubbles will form. F, Make sure to leave a central round area large enough to allow confocal imaging. G, If too much glue is applied, the glue will expand blocking the view. H, When ready to image cells with confocal microscope, add a small amount of HBSSCM to prevent dehydration and then a single bead, a small section of tissue or a rodent IVD