TABLE 2.
List of molecular markers and TaqMan probes used for their analysis
| Category | Gene name | Gene symbol | Assay ID | Function |
|---|---|---|---|---|
| Inflammation | nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105/p50 | Nfkb1 | Mm00476361_m1 | NFKB subunit activated in response to inflammation, produces the p50 and p105 subunits 66 |
| v‐rel reticuloendotheliosis viral oncogene homolog A, p65 | Rela | Mm00501346_m1 | NFKB subunit activated in response to inflammation, produces the p65 subunit 67 , 68 | |
| Glial fibrillary acidic protein | Gfap | Mm01253033_m1 | Marker of glial activation in response to inflammation 69 | |
| Interleukin 6 | Il6 | Mm00446190_m1 | Secreted in acute phase of inflammation, important for the transition to chronic inflammation and pain 70 | |
| Interleukin 1 beta | Il1b | Mm00434228_m1 | Increased bilaterally in DRGs following peripheral inflammation 71 | |
| Tumor necrosis factor | Tnf | Mm00443258_m1 | Inflammatory mediator, induces ectopic discharge in c‐fibers 72 | |
| Cytochrome oxidase subunit 2 (COX2) | Cox2 | Mm03294838_g1 | Inflammatory mediator, produced in spinal cord and DRGs following inflammation 73 , 74 | |
| Nociception | Calcitonin Related Polypeptide Alpha | Calca | Mm00801463_g1 | Nociceptive neuropeptide involved in the transmission of and sensitization to noxious stimuli from PNS to CNS 34 , 75 |
| Tachykinin Precursor 1 | Tac1 | Mm01160362_m1 | TAC1 protein is a nociceptive neuropeptide involved in the transmission of noxious stimuli from PNS to CNS 76 , 77 | |
| Receptor Activity Modifying Protein 1 | Ramp1 | Mm00489796_m1 | Receptor that allows CALCA to bind to the calcitonin‐receptor‐like receptor in the SCDH 78 , 79 | |
| Tachykinin Receptor 1 | Tacr1 | Mm00436892_m1 | Receptor in spinal cord that binds TAC1 to transmit nociceptive input in chronic pain conditions 80 | |
| Transient Receptor Potential Cation Channel Subfamily A Member 1 | Trpa1 | Mm01227437_m1 | Cold sensing ion channel involved in cold sensitivity in both inflammation and nerve injury 81 | |
| Nerve Injury | Neuropeptide Y | Npy | Mm01410146_m1 | Neuropeptide induced following nerve injury 82 increases firing rates of DRGs 83 |
| Activating Transcription Factor 3 | Atf3 | Mm00476033_m1 | Transcription factor induced following nerve injury 21 | |
| Neurotrophic factors | Brain Derived Neurotrophic Factor | Bdnf | Mm04230607_s1 | Expression in DRGs contributes to chronic pain 84 |
| Internal Control | Glyceraldehyde‐3‐Phosphate Dehydrogenase | Gapdh | Mm99999915_g1 | Stable housekeeping gene in the DRGs 25 |