TABLE 4.

Relative gene expression in L4‐6 DRGs and lumbar SCDH from mice at specified age. P‐values are based on Dunnett's post‐hoc test. Raw Ct value of Gapdh included in last column

			Relative gene expression to Gapdh (S.E.M)n = 5–8 per cohort						P‐value
	Tissue	Gene	12 months		18 months		24 months		12 vs 24	18 vs 24
Nociception	DRG	Calca	0.549	(0.083)	0.952	(0.189)	1.067	(0.207)	0.0747	0.8455
	SCDH	Calca	0.071	(0.009)	0.104	(0.022)	0.163	(0.0388)	0.0543	0.2410
	SCDH	Ramp1	0.033	(6.51×10−3)	0.065	(3.52×10−2)	0.091	(3.22×10−2)	0.2796	0.7445
	DRG	Tac1	3.05×10−4	(1.22×10−4)	8.43×10−4	(3.22×10−4)	5.61×10−4	(4.31×10−4)	0.7883	0.4855
	SCDH	Tac1	4.96 ×10−3	(1.48×10−3)	6.×10−3	(8.15×10−3)	8.15×10−3	(2.74×10−3)	0.6308	0.8107
	SCDH	Tacr1	9.30 ×10−3	(4.71×10−3)	7.06×10−3	(2.75×10−3)	4.55×10−3	(2.85×10−3)	0.5561	0.8387
Cold	DRG	Trpa1	0.012	(2.31×10−2)	0.025	(7.26×10−2)	0.026	(4.87×10−2)	0.1037	0.9590
Inflammation	DRG	Nfkb1	3.03 ×10−3	(×10−3)	3.99×10−3	(5.70×10−4)	8.31×10−3	(9.21×10−4)	0.0002	0.0016
	DRG	Rela	0.030	(3.33×10−3)	0.048	(7.80×10−3)	0.059	(0.010)	0.0239	0.4908
	DRG	Il1b	1.21 ×10−3	(4.80×10−4)	5.95×10−4	(1.38×10−4)	1.31×10−3	(1.78×10−4)	0.9997	0.5542
	DRG	Il6	5.79 ×10−5	(1.72×10−5)	4.81×10−5	(2.10×10−5)	1.03×10−4	(3.73×10−5)	0.2117	0.0636
	DRG	Tnf	2.29 ×10−10	(4.75×10−11)	4.74×10−10	(1.65×10−10)	2.05×10−10	(1.95×10−11)	0.9755	0.2230
	DRG	Gfap	5.01 ×10−3	(1.03×10−3)	7.90×10−3	(4.17×10−4)	5.24×10−3	(1.59×10−3)	0.9899	0.3401
	DRG	Cox	1.52	(0.178)	2.24	(0.307)	1.67	(0.185)	0.8906	0.5123
Nerve injury	DRG	Atf3	2.11 ×10−3	(4.84×10−4)	3.81×10−3	(7.23×10−4)	5.99×10−3	(8.23×10−4)	0.0030	0.0835
	DRG	Npy	3.81 ×10−4	(1.19×10−4)	8.95×10−4	(2.67×10−4)	8.10×10−3	(9.13×10−5)	0.0567	0.8661
Nerve growth	DRG	Bdnf	5.18 ×10−4	(1.05×10−4)	9.14×10−4	(1.98×10−4)	1.20×10−3	(2.35×10−4)	0.0437	0.4948
Ct of Gapdh	DRG	Gapdh	23.47	(0.4380)	23.47	(0.5712)	23.25	(0.5931)	0.9999	0.9999