Table 3.
Origin and characterization of Aspergillus fumigatus isolates using CYP51A sequencing, CSP typing, and STRAf profiling.
| Isolatea | Origin/year | CYP51A | CSP | STRAf marker profile | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2A | 2B | 2C | 3A | 3B | 3C | 4A | 4B | 4C | ||||
| WT-NL | Netherlands, before 2014 | Wt | t01 | - | - | - | - | - | - | - | - | - |
| PG3-3 | UK, 2015 | Wt | t01 | 27 | 18 | 15 | 8 | 11 | 31 | 26 | 10 | 8 |
| AF65 | UK, 1997 | Wt | t02 | 14 | 20 | 12 | 35 | 9 | 10 | 8 | 10 | 21 |
| PG2-10 | UK, 2015 | Wt | t04B | 18 | 12 | 8 | 27 | 10 | 18 | 9 | 9 | 5 |
| BB1-2.2-B1 | UK, 2015 | Wt | t18A | 13 | 21 | 11 | 31 | 27 | 8 | 10 | 9 | 8 |
| BB6-8-B1 | UK, 2015 | D262Y | t03 | 20 | 19 | 8 | 36 | 14 | 22 | 9 | 9 | 5 |
| SS5-2A | Austria, 2015 | D262Y | t05 | 18 | 12 | 9 | 10 | 10 | 12 | 8 | 10 | 7 |
| PG2-6 | UK, 2015 | D262Y | t05 | 15 | 20 | 9 | 10 | 10 | 6 | 8 | 10 | 10 |
| BB1-3-B9 | UK, 2015 | F46Y/M172V/E427K | t02* | 10 | 14 | 10 | 17 | 13 | 8 | 7 | 5 | 6 |
| G1-A9 | Germany, 2016 | F46Y/M172V/E427K | t02* | 10 | 14 | 10 | 17 | 13 | 8 | 7 | 5 | 6 |
| SS5-7C | Austria, 2016 | F46Y/M172V/E427K | t08 | 10 | 16 | 10 | 17 | 13 | 20 | 7 | 5 | 6 |
| UK2-B4 | UK, 2016 | F46Y/M172V/E427K | t08 | 10 | 15 | 10 | 26 | 11 | 8 | 7 | 5 | 5 |
| F5-C6 | France, 2016 | F46Y/M172V/E427K | t15 | 10 | 14 | 10 | 17 | 13 | 13.1 | 7 | 5 | 5 |
| BB6-8-C7 | UK, 2015 | F46Y/M172V/E427K | t19 | 10 | 15 | 10 | 19 | 12 | 12 | 7 | 5 | 6 |
| AF293 | UK, 1993 | F46Y/M172V/N284T/ D255E/E427K | t06A | 26 | 18 | 18 | 46 | 21 | 23 | 11 | 10 | 8 |
| TR34-NL | Netherlands, before 2014 | TR34/L98H | - | - | - | - | - | - | - | - | - | - |
| UK5-B5 | UK, 2016 | TR34/L98H | t02 | 14 | 20 | 8 | 31 | 9 | 10 | 8 | 10 | 11 |
| BTCa-1 | Netherlands, 2018 | TR34/L98H | t02 | - | - | - | - | - | - | - | - | - |
| HPPb-1 | UK, 2018 | TR34/L98H | t02 | 25 | 20 | 18 | 31 | 9 | 10 | 10 | 14 | 5 |
| 08-19-02-10 | Netherlands, 2008 | TR34/L98H | t04B | 25 | 24 | 12 | 84 | 9 | 9 | 8 | 10 | 11 |
| RN8-18 | UK, 2016 | TR34/L98H | t04B | 25 | 24 | 12 | 84 | 9 | 9 | 8 | 10 | 11 |
| SS10-6A | Netherlands, 2016 | TR34/L98H | t11 | 20 | 21 | 16 | 16 | 12 | 7 | 16 3 | 24 | 33 |
| SS8-7A | Belgium, 2016 | TR34/L98H | t11 | 20 | 21 | 16 | 77 | 12 | 11 | 16 3 | 11 | 21 |
| F1-C5 | France, 2016 | TR34/L98H | t11 | - | - | - | - | - | - | - | - | - |
| BTTa-1 | Netherlands, 2018 | TR46/Y121F/M172I/ T289A/G448S | t01 | 26 | 21 | 12 | 26 | 10 | 6 | 13 | 9 | 20 |
| TR46-NL | Netherlands, before 2014 | TR46/Y121F/T289A | - | - | - | - | - | - | - | - | - | - |
| WN19-3 | UK, 2016 | TR46/Y121F/T289A | t01 | 10 | 19 | 12 | 45 | 9 | 50 | 8 | 10 | 10 |
| WN28-6 | UK, 2016 | TR46/Y121F/T289A | t01 | 20 | 20 | 14 | 49 | 9 | 36 | 8 | 9 | 9 |
| RS3-3 | UK, 2016 | TR46/Y121F/T289A | t02 | 10 | 20 | 8 | 42 | 9 | 10 | 12 | 10 | 20 |
| BKCb-1 | Netherlands, 2018 | TR46/Y121F/T289A/ S363P/I364V/G448S | t01 | 26 | 20 | 12 | 43 | 11 | 11 | 12 | 9 | 9 |
Isolates grouped according to CYP51A sequence and CSP type.
a
AF65 and AF293 are clinical isolates; -, unknown or not determined.