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. 2020 Jul 18;15:53–63. doi: 10.1016/j.reth.2020.06.001

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© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Neurosphere culture of HUCB p75NTR⁺ cells. Freshly isolated p75NTR⁺ and p75NTR⁻ cell fractions were cultured in a neurosphere culture condition or in a mesenchymal stem cell (MCS) culture condition. (a) Representative images of each cell fraction. (b) Bromodeoxyuridine (BrdU) incorporation in p75NTR⁺ cell-derived spheres. (c) Immunocytochemical analysis of p75NTR⁺ cell-derived spheres. Arrows represent p75NTR⁺ small spheres. (d) Representative images of secondary and tertiary spheres. Scale bars represent 50 μm.