Fig. 2.

(A) Normal Human Epidermal Keratinocytes (NHEK) cultured on temperature-responsive culture inserts were harvested as a cell sheet after incubation at 20 °C for 30 min. (B) Ex vivo porcine ESD model (1) Utilizing freshly excised slaughterhouse porcine tissue. (2) A longitudinal incision with Cooper scissors is made from the oral side of the esophagus to the gastric side (3,4) Dissection of about 10 cm of submucosa with Cooper scissors. (C) Transplantation of untreated and expanded cell sheets (control group and expanded group). (D) Expanded group (1) cell sheet is placed on the deflated balloon device. (2) Inflate the balloon for 30 s by injecting 20 cc of air to inflate then deflate repeatedly in sequence 3 times total.