Fig. 1.

Comparison of chondrogenesis efficacy between various HA gels. Chondrocytes differentiated 80,000 cells of hMSCs were seeded either on matrigel-coated wells on 60 μL of hyaluronan gel (Euflexxa®, Orthovisc®, Supartz® and Synvisc®) or without HA (control) in the wells of 96-well plates and incubated in chondrogenic medium for 15 days. (a) The expression of Type Ⅰ collagen, Type Ⅱ collagen and MMP-13 was measured by immunoblot analysis. (b) The expression of Type Ⅰ collagen was only shown in MSC group. (c) Type Ⅱ collagen expression was significantly elevated in all treatment groups except Supartz®. There was no statistical difference among Euflexxa®, Orthovisc® and Supartz®, (α, β, γ P > 0.05) (d) MMP-13 expression was highly elevated in all HA treatment groups.