Figure 3.

Chronic stress fully increased the phosphorylation level of Cofilin in the medial prefrontal cortex (mPFC) of mice. Representative western blotting images indicate that the chronic unpredictable mild stress (CUMS)-treated (A), chronic restraint stress (CRS)-treated (B), and chronic social defeat stress (CSDS)-treated (C) mice all exhibited significantly higher level of pCofilin in the mPFC but not the hippocampus than the control mice (n = 5). The level of total Cofilin in both the mPFC and hippocampus remained unchanged among all groups (n = 5). All results are expressed as the means ± SEM; **P < .01; n.s., no significance. The comparisons were made using t test.