Figure 3.

mRNA expression of core circadian clock genes changes slightly during aging

(A and B) Expression of the four core clock genes, bmal1, clockb, cry1b, and per3, in the whole brain of young (6-week-old) and old (14-week-old) killifish under LD (A) and DD (B). The expression of each gene was normalized to the expression of insr, a gene that shows constant expression during aging. Data are expressed as the mean ± SD of five fish at each time point. ∗p<0.05, ∗∗p<0.01, ∗∗∗p<0.001 and ∗∗∗∗p<0.0001 after two-way ANOVA followed by the Sidak's multiple comparison test.

See also Figures S3 and S4 and Table S1.