Figure 5.

The amount and function of the Bmal1/Clockb heterodimer are retained in old killifish brain

(A) Bmal1/Clockb heterodimer formation throughout the day in the brain of young and old fish. Co-immunoprecipitated (co-IPed) Clockb was normalized against IPed-Bmal1 for each trial. Data are presented as the mean ± SD from three replicates.

(B and C) Binding affinity of Bmal1 to the promoter regions of cry1b (B) and per3 (C) in the brains of young and old fish. Schematic diagrams of the promoter regions of cry1b and per3 are shown along with the amplicon positions. E-box, red bar; gray box, 5′-UTR; black box, protein coding sequence; solid arrow, start codon; pale blue bar, amplicon position. Data are presented as the mean ± SE from three replicates. Asterisks indicate ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗∗p < 0.0001 after two-way ANOVA followed by the Tukey's multiple comparison test.

See also Figure S7.