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. 2020 Dec 9;24(1):101915. doi: 10.1016/j.isci.2020.101915

Figure 7.

Figure 7

Relationship between TPI, HIF-1, and glycolysis

(A) Influence of TPI inhibitor on TPI activity in shrimp. Shrimp were injected with TPI inhibitor phenazine (100 μM). Twenty-four hours later, the enzymatic activity of TPI in the hemocytes of shrimp was examined. DMSO was used as a control. The error bars denoted the means ± standard deviations of three independent experiments (∗∗p < 0.01).

(B) Impact of the inhibition of TPI activity on glycolysis of shrimp. At 24 hr after the injection of TPI inhibitor, the contents of glucose and lactate in the hemocytes of shrimp were examined. The error bars represented the means ± standard deviations of three independent experiments (∗∗p < 0.01).

(C) Effects of the suppression of TPI activity on the HIF-1 expression in shrimp. At 24 hr after the injection of TPI inhibitor, Western blot analysis was conducted to examine the expression level of HIF-1 protein in shrimp hemocytes. β-actin was used as a control.

(D) Evaluation of the impact of HIF-1 silencing on the expression of TPI in shrimp. Shrimp were injected with HIF-1-siRNA. As a control, HIF-1-siRNA-scrambled was included in the injection. At 36 hr after injection, the expression level of TPI protein in the hemocytes of shrimp was analyzed by Western blot. β-actin was used as a control.