Fig. 2.

NO-controlled release from Ag₂S@BSA-SNO nanoparticles and in vitro therapeutic efficiency of X-ray enhanced by Ag₂S@BSA-SNO nanoparticles. a IR images of Ag₂S@BSA and Ag₂S@BSA-SNO solution under 808 nm laser irradiation. b Temperature change in Ag₂S@BSA-SNO solution during 808 nm laser irradiation with different concentrations. c UV–Vis–NIR spectra of Ag₂S@BSA-SNO solution with different concentrations. d Temperature variations of Ag₂S@BSA-SNO under irradiation by 808 nm laser (1.5 W cm⁻²). e PA intensity of Ag₂S@BSA-SNO solution at near-infrared region. f PA signal of Ag₂S@BSA-SNO solution (at 730 nm laser). g NO released under different temperatures. h 808 nm laser irradiation trigger NO release (red triangle means NIR stimulation). i Cell cloning statistics. The temperature caused by 808 nm laser is around 42 °C, while the X-ray dose is 6 Gy. j, k Statistics and confocal laser scanning microscopy image of DNA damage staining after photothermal and irradiation therapeutics. Red: γ-H2AX signal (dsDNA damage staining); blue: DAPI (nuclear staining). Data are means ± SEM (n ≥ 3). P value is based on student’s t test, with ****P < 0.0001. (Color figure online)