Figure 3.

b-AP15 and structurally related compound induce accumulation of polyubiquitinated proteins on proteasomes. Lysates from cells exposed to different compounds were fractionated by glycerol gradient centrifugation, and fractions were collected and processed for immunoblotting. Compounds CID1381237, CID2247230, and CID2175947 were identified in a cell-based screen for compounds stimulate accumulation of Ub^G76V-YFP in exposed cells.⁵⁷ CID 2175947 (CB826 in ref (57)) is also known as MCB-613 and has been described to stimulate steroid receptor coactivator activity and to be preferentially cytotoxic to cancer cell lines.²³ CID1381237 is also known as A9⁷⁶ (Figure 1) and C8.⁶³ Data reproduced from ref (57), which licensed under CC BY 4.0.