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. 2020 Dec 3;83(12):3614–3622. doi: 10.1021/acs.jnatprod.0c00776

Figure 3.

Figure 3

(A–C) Changes in TEER of IPEC-J2 cell monolayers incubated from day 7 with medium containing LSH at concentrations of 100 and 500 μg/mL, castalagin (1), vescalagin (2), salicarinin A (3), or salicarinin B (4) at a 20 μM concentration. The data are representative for three independent experiments assayed in triplicate (LSH and 1) or one experiment assayed in triplicate (2, 3, and 4). (D–F) Respective changes in tight junction protein (TJ) expression using Western blot performed at day 13. Western blots and calculations are provided in Table S1 (Supporting Information). Spearman correlation analysis between TEER and TJ levels are provided in Table S2 (Supporting Information).