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. 2020 Dec 15;11:622907. doi: 10.3389/fmicb.2020.622907

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Copyright © 2020 Barrado-Gil, Del Puerto, Muñoz-Moreno, Galindo, Cuesta-Geijo, Urquiza, Nistal-Villán, Maluquer de Motes and Alonso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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UBCv1 functions as an E2 conjugating enzyme. (A) Analysis of the interaction between UBCv1/UBCv1C85A and ubiquitin in an ubiquitylation assay. (B) Representative images of the immunoprecipitation of HEK293 co-expressing Flag-UBCv1 plasmid or (C) Flag-UBCv1C85A and one of the following HA-Ub variations: HA-UbK48, HA-UbK63, HA-UbK48R, or HAUb-K63R. Spliced and grouped images from blots in boxes in (A–C) are shown in full in Supplementary Figure S1. Western blotting against HA and Flag tags was used for detection and whole-cell lysates (WCL) were loaded as transfection rate controls.