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. 2020 Dec 15;11:622907. doi: 10.3389/fmicb.2020.622907

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Copyright © 2020 Barrado-Gil, Del Puerto, Muñoz-Moreno, Galindo, Cuesta-Geijo, Urquiza, Nistal-Villán, Maluquer de Motes and Alonso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ASFV UBCv1 binds cellular translation factor eIF4E. (A) IP assay of UBCv1 protein and eIF4E, analyzed by western blot. The interaction between both proteins was detected using α-FLAG and α-HA antibodies and transfection rates and expression checked in WCL samples. (B) Representative confocal microscopy images of cells transfected with HA-eIF4E for 12 h and infected with Ba71V for 16 h. Colocalization for UBCv1 (red) and HA (eIF4E, green) was observed as a yellow signal. VFs of infected cells stained for viral protein p72 (blue). Bar = 20μm (C) Cap-complex immunoprecipitation using the cap-analog m7GTP-Sepharose of Ba71V infected cells. eIF4E bound to the cap-complex but not UBCv1, which bound eIF4E independently from the cap-complex.