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. Author manuscript; available in PMC: 2020 Dec 29.
Published in final edited form as: Cell Rep. 2020 Nov 24;33(8):108433. doi: 10.1016/j.celrep.2020.108433

Figure 6. Prdm1 and Maf Synergistically Regulate IL-10 in All T Helper Cells.

Figure 6.

(A) Enrichment of Prdm1 and Maf mRNA in in-vitro-generated T helper cells validated by qPCR.

(B) ChIP-seq of Maf in Th17 cells and Prdm1 in tissue-resident memory T cells aligned with ATAC-seq data of Tr1 cells differentiated in vitro at 72 h.

(C) Luciferase activity in 293T cells transfected with Il10 luciferase reporters along with constructs encoding Prdm1, Maf, or both. n = 3.

(D) T helper cells differentiated in vitro were transduced with two retroviruses expressing Prdm1 and Maf, respectively. IL-10 expression in control cells, Prdm1-overexpressing cells, Maf-overexpressing cells, and cells overexpressing Prdm1 and Maf was measured by flow cytometry 48 h after transduction.