Fig. 3.

Effect of TST and SAHA in the acetylation levels of histone H3 in T. gondii after 48 h of treatment. A-E Immunofluorescence analysis of the acetylated H3 labeling of tachyzoites treated with SAHA and TST. Tachyzoites were labeled with anti-acetylated H3 (red), anti-SAG-1 (green), and DAPI (blue). F Western blot labeled with anti-H3 and anti-acetylated-H3/Lys 9/14. G The relativization of rH3ac/rH3. Statistical analysis was performed using one-way ANOVA and Dunnett's multiple comparisons test. F** 0.0048, P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)