Fig. 4.

Effect of TST and SAHA on parasite microtubules. A-B Immunofluorescence and Western blotting labeled with α-tubulin. C-D labeled with α-acetylated tubulin after TST 1 μM and SAHA 0.1 μM treatment. Immunofluorescence assays were performed using RH-RFP (red fluorescence protein) tachyzoites, after 24 h treatment. 5 μm scale bar. Western blots were performed after 48 h treatment. E Quantification of α-tubulin and acetylated α-tubulin in percentage. Statistical analyses were performed using One-Way ANOVA, Bonferroni multiple comparison test, B **0.0043, P < 0.05; D ***0.0009, P < 0.05, n3. F Ratio of acetylated/total tubulin. Three independent experiments were performed in triplicate. Statistical analysis was performed using One-Way ANOVA, Bonferroni multiple comparison test, B **0.0043, P < 0.05; D ***0.0009, P < 0.05. NS- not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)