Fig. 6.

Effect of TST and SAHA on parasite apicoplast by Immunofluorescence. Effect of SAHA and TST. RH-ACP-YFP tachyzoites were treated with TST (1 μM) or SAHA (0.1 μM) for 24 h were labeled with anti-SAG1 and anti-Centrin1. Control parasites presented normal morphology and only one centrosome and apicoplast. Treatment with TST and SAHA affected apicoplast partition and led to organelle fragmentation (white arrows). 2 μm scale bar. B 3D SR-SIM projection of NDHF fibroblasts infected with the RH strain. Treatment with both compounds led to the interruption of daughter cells' budding process. Several centrosomes randomly dispersed in the round masses of damaged parasites are clearly seen (arrowheads). 2 μm scale bar.