Fig. 7.

Effect of TST and SAHA on parasite inner membrane complex. Immunofluorescence analysis showing NDHF fibroblasts infected with RH strain treated with 1 μM TST or 0.1 μM SAHA for 24 h. Treated cells were labeled with anti-IMC1. Control tachyzoites formed characteristic rosettes with completely assembled IMC (bracket), whereas the IMC in TST treated parasites was drastically disorganized (white arrow). SAHA treatment revealed incomplete IMC assembly (arrowhead) and both compounds led to the formation of a mass of damaged parasites (asterisk). 2 μm scale bar.