Fig. 8.

Transmission electron microscopy of parasites treated with 3 μM TST or 0.2 μM SAHA for 12, 24 and 48 h. A, D and G Control parasites with typical ultrastructure after 12, 24 and 48 h. B–C Treatment with both compounds led to damages in the endodyogeny process and formation of large parasites. B, E and H TST-treated parasites presented mitochondria with disrupted crista and vacuolization of the cytoplasm. C After SAHA treatment, tachyzoites presented apicoplast with unusual morphology, apparent fragmentation (inset) and myelin-like structures in F (inset). I SAHA led to impaired new parasite individualization and consequently, the formation of a mass of parasites connected by the inner membrane complex (arrow inset). Nucleus (N), Mitochondria (M), Apicoplast (Ap), Vacuole (V) Parasitophorous Vacuole (PV). Scale bars: A, B, C, F and I = 1 μm; D = 2 μm; E = 0.5 μm; G, H = 2 μm. C, F and I insets = 1 μm.