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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Oct 27;520:111071. doi: 10.1016/j.mce.2020.111071

Figure 2: Pde11a mRNA integrity in Pde11a−/− sample and its expression in the studied mice.

Figure 2:

(A) Products of RT-PCR using primers spanning different regions along the Pde11a transcript of KO and Pde11a+/+ mice: A-1) primer spaning exons 9–10 and exons 12–13; A-2) primers spaning exons 14–15 and exons 18–19; A-3) primers spanning exons 1–2 and 10–11: Amplicons were analyzed by gel electrophoresis on a 2% agarose gel. Amplification in Pde11a−/− mice indicates that Pde11a−/− RNA transcript is intact from exon 1 throught 19, is not degraded and incorporate the LacZ-Neo cassette (B) qRT-PCR levels of Pde11a in adrenal, brain, testis and lungs from heterozygote (Het) Pde11a+/, and knock-out (KO) Pde11a−/− mice against Pde11a+/+ mice; clearly Pde11a mRNA is decreased in the genetically modified animals but not absent in the KO mice, *p value for KO sample 0.05, **p value KO= 0.001; (C) Protein expression of Pde11a, assessed by western blot (WB) analysis, in testis amd adrenal, as an example and quantified in testis by optical density of WB bands; once again, Pde11a protein in testis of the KO mouse is present and is lower than in the Pde11a+/+ animal but not absent. (D) Pde11a immunostainig (red) merged with nuclear staining (DAPI – blue) in Pde11a+/+ and Pde11a−/− adrenals, immunoractivity is rubust in zona fasciculata of both genotypes but reduced in Pde11a−/− adrenal. Abbreviations: Br, brain, Ad, adrenal, T, testis, M, medulla, ZF zona fasciculata, ZG, zona glumerolosa.